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viability dye  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences viability dye
    Viability Dye, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ghost+dye+violet+450+viability+dye/Ghost+Dye+Violet+450/pmc13012245-147-19-21
    Average 94 stars, based on 112 article reviews
    viability dye - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Immunomodulatory effects of Purion processed human amniotic membrane allografts in vitro.
    Article Snippet: .. For polarization experiments, macrophages were stained for 15 min at 4 °C in the dark with FACS buffer containing Fc Receptor Binding Inhibitor Antibody (eBioscience) and Ghost Dye Violet 450 viability dye (Tonbo Biosciences). .. After 15 min, cells were stained in accordance with manufacturer’s instructions with antibodies against the following cell surface markers: pro-inflammatory markers CD80 (BV711), CD86 (PE-Cy5), and pro-repair markers CD163 (BV785), CD36 (AF700), and CD206 (PE-Dazzle 594) (BioLegend).

    Article Title: Cancer-associated fibroblast compositions change with breast cancer progression linking the ratio of S100A4 + and PDPN + CAFs to clinical outcome.
    Article Snippet: .. E0771-mCherry tumors were stained with Ghost-Dye-Violet 450 viability dye (TONBO), CD45−BV711, PDPN–APC, Ly6C-PerCP/Cy5.5 and then fixed, permeabilized and intracellularly stained with α-SMA–FITC. ..

    Article Title: Immunomodulatory effects of Purion processed human amniotic membrane allografts in vitro
    Article Snippet: .. * p < 0.05 indicates a statistically significant difference between treatment groups and Monocyte (LPS) For polarization experiments, macrophages were stained for 15 min at 4 °C in the dark with FACS buffer containing F c Receptor Binding Inhibitor Antibody (eBioscience) and Ghost Dye Violet 450 viability dye (Tonbo Biosciences). .. After 15 min, cells were stained in accordance with manufacturer’s instructions with antibodies against the following cell surface markers: pro-inflammatory markers CD80 (BV711), CD86 (PE-Cy5), and pro-repair markers CD163 (BV785), CD36 (AF700), and CD206 (PE-Dazzle 594) (BioLegend).

    FACS:

    Article Title: Immunomodulatory effects of Purion processed human amniotic membrane allografts in vitro.
    Article Snippet: .. For polarization experiments, macrophages were stained for 15 min at 4 °C in the dark with FACS buffer containing Fc Receptor Binding Inhibitor Antibody (eBioscience) and Ghost Dye Violet 450 viability dye (Tonbo Biosciences). .. After 15 min, cells were stained in accordance with manufacturer’s instructions with antibodies against the following cell surface markers: pro-inflammatory markers CD80 (BV711), CD86 (PE-Cy5), and pro-repair markers CD163 (BV785), CD36 (AF700), and CD206 (PE-Dazzle 594) (BioLegend).

    Article Title: Immunomodulatory effects of Purion processed human amniotic membrane allografts in vitro
    Article Snippet: .. * p < 0.05 indicates a statistically significant difference between treatment groups and Monocyte (LPS) For polarization experiments, macrophages were stained for 15 min at 4 °C in the dark with FACS buffer containing F c Receptor Binding Inhibitor Antibody (eBioscience) and Ghost Dye Violet 450 viability dye (Tonbo Biosciences). .. After 15 min, cells were stained in accordance with manufacturer’s instructions with antibodies against the following cell surface markers: pro-inflammatory markers CD80 (BV711), CD86 (PE-Cy5), and pro-repair markers CD163 (BV785), CD36 (AF700), and CD206 (PE-Dazzle 594) (BioLegend).

    Binding Assay:

    Article Title: Immunomodulatory effects of Purion processed human amniotic membrane allografts in vitro.
    Article Snippet: .. For polarization experiments, macrophages were stained for 15 min at 4 °C in the dark with FACS buffer containing Fc Receptor Binding Inhibitor Antibody (eBioscience) and Ghost Dye Violet 450 viability dye (Tonbo Biosciences). .. After 15 min, cells were stained in accordance with manufacturer’s instructions with antibodies against the following cell surface markers: pro-inflammatory markers CD80 (BV711), CD86 (PE-Cy5), and pro-repair markers CD163 (BV785), CD36 (AF700), and CD206 (PE-Dazzle 594) (BioLegend).

    Article Title: Immunomodulatory effects of Purion processed human amniotic membrane allografts in vitro
    Article Snippet: .. * p < 0.05 indicates a statistically significant difference between treatment groups and Monocyte (LPS) For polarization experiments, macrophages were stained for 15 min at 4 °C in the dark with FACS buffer containing F c Receptor Binding Inhibitor Antibody (eBioscience) and Ghost Dye Violet 450 viability dye (Tonbo Biosciences). .. After 15 min, cells were stained in accordance with manufacturer’s instructions with antibodies against the following cell surface markers: pro-inflammatory markers CD80 (BV711), CD86 (PE-Cy5), and pro-repair markers CD163 (BV785), CD36 (AF700), and CD206 (PE-Dazzle 594) (BioLegend).

    Incubation:

    Article Title: Heat Shock Factor 1-dependent extracellular matrix remodeling mediates the transition from chronic intestinal inflammation to colon cancer
    Article Snippet: .. WT and Hsf1 null colons were washed through a strainer with PBS, dissociated by HBSS with 5 mM EDTA and 10 mM Hepes for 20 min, incubated for 10 min on ice with Ghost-Dye-Violet 450 viability dye (TONBO), washed and resuspended with MACS buffer (PBS (calcium and magnesium free), 0.05% BSA and 0.04% EDTA 0.5 M pH 8) for 10 min on ice with anti-mouse CD16/32 antibody. ..

    Magnetic Cell Separation:

    Article Title: Heat Shock Factor 1-dependent extracellular matrix remodeling mediates the transition from chronic intestinal inflammation to colon cancer
    Article Snippet: .. WT and Hsf1 null colons were washed through a strainer with PBS, dissociated by HBSS with 5 mM EDTA and 10 mM Hepes for 20 min, incubated for 10 min on ice with Ghost-Dye-Violet 450 viability dye (TONBO), washed and resuspended with MACS buffer (PBS (calcium and magnesium free), 0.05% BSA and 0.04% EDTA 0.5 M pH 8) for 10 min on ice with anti-mouse CD16/32 antibody. ..

    other:

    Article Title: TCF1 lo CD8 T cells proliferate and persist autonomously in tumors
    Article Snippet: 4′,6-diamidino-2-phenylindole (DAPI), BioLegend, Cat# 422801, Cas-No: 47165-04-8; Ghost Dye Violet 450 Viability Dye, Tonbo/Cytek, Cat# 13-0863; Ghost Dye Red 710 Viability Dye, Tonbo/Cytek, Cat# 13-0871; Ghost Dye Red 780 Viability Dye,Tonbo/Cytek, Cat# 13-0865.



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